B-1a cells are a unique subset of B cells that are phenotypically and functionally distinguishable from conventional (B-2) cells. Here we detail methods to analyze signaling events in B-1a cells. We demonstrate by flow cytometry and Western blot that mouse peritoneal B-1a but not splenic B-2 cells have constitutive activation of signal transducers and activators of transcription-3 (Stat3) and extracellular signal-regulated kinase (Erk).
CITATION STYLE
Huo, J., Huang, Y., Xu, S., & Lam, K. P. (2017). Analysis of signaling events in B-1a cells. In Methods in Molecular Biology (Vol. 1643, pp. 75–83). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7180-0_6
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