We will be manufacturing non-intact reconstructed beef patties, inoculating it with E.coli O157:H7 and aerobically storing in a PVC film-covered foam tray. We will evaluate the cooking inactivation activity of E. coli O157:H7 in beef patties with various cooking time. We will be enumerating survivals on support and selective medium and using USDA-IPMP 2013 software to analyze data and determine the fitted model. Finally, presumptive E. coli O157:H7 survivals will be identified using Enterotube II biochemistry test and latex agglutination test. In this chapter, it requires students to finish part of the work independently
CITATION STYLE
Shen, C., & Zhang, Y. (2017). Thermal Inactivation of Escherichia coli O157:H7 in Non-intact Reconstructed Beef Patties. In Food Microbiology Laboratory for the Food Science Student (pp. 51–57). Springer International Publishing. https://doi.org/10.1007/978-3-319-58371-6_9
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