Functionalization of the genome is carried out by proteins that bind to DNA to regulate gene expression. Since this process is highly dynamic, context-dependent, and rarely performed by single proteins alone, we here describe ChIP-SICAP to identify proteins that co-localize with a protein of interest on the genome. Benefiting from its nature as a dual purification approach via ChIP and DNA biotinylation, ChIP-SICAP distinguishes genuine chromatin-binders and is uniquely placed to identify novel players in genome regulation.
CITATION STYLE
Rafiee, M. R., & Krijgsveld, J. (2021). Using ChIP-SICAP to Identify Proteins That Co-localize in Chromatin. In Methods in Molecular Biology (Vol. 2351, pp. 275–288). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1597-3_15
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