Epitope Analysis Using Synthetic Peptide Repertoires Prepared by SPOT Synthesis Technology

Abstract

Abbreviations AA Amino acid AAI Antibody-antigen interaction Abu a-Aminobutyric acid Acm Acetyl-aminomethyl AP Alkaline phosphatase BCIP 5-bromo-4-chloro-3-indolylphosphate p-toluidine salt BPB Bromophenol blue BSA Bovine serum albumine CBS Citrate-buffered saline CDS Colour developing solution DCM Dichloromethane DIC N,N 0-Diisopropylcarbodiimide ECL Enhanced chemiluminescence FITC Fluoresceine isothiocyanate HOBt N-Hydroxybenzotriazole HRP Horse radish peroxidase kd Kilo dalton MBS Membrane blocking solution MTBE tert-Butyl methylether MTT 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide NBT Nitro blue tetrazolium chloride monohydrate NMP N-Methyl-2-pyrrolidinone PBS Phosphate-buffered saline