RNAi is a powerful tool that can be used to probe gene function as well as for therapeutic intervention. We describe a workflow and methods to identify screen and select potent and specific siRNAs in vitro and in vivo using qPCR-based methods as well as an AAT activity assay. We apply these techniques to a set of siRNAs targeting rat AAT, and use this set to exemplify the cell-based and in vivo data that can be generated using these methods.
CITATION STYLE
Milstein, S., Qian, K., Zhang, L., & Sehgal, A. (2017). Methods to identify and characterize siRNAs targeting serpin A1 in vitro and in vivo using RNA interference. In Methods in Molecular Biology (Vol. 1639, pp. 115–126). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7163-3_11
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