Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria ‘from domain to strain’. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb)(BIGSdb) allows the scalable organization and analysis of whole-genome sequence data.
CITATION STYLE
Tworzydlo, W., & Bilinski, S. M. (2019). Evo-Devo: Non-model species in cell and developmental biology. Acta Derm Venereol (Vol. 93, p. 550).
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