Optimization of Medium and Incubation Time in the Production of Antibacterial Compounds by S treptomyces sp. SA404

Abstract

Streptomyces sp. SA404 was isolated from the mangrove rhizosphere in Segara Anakan Cilacap known for its production of antibacterial compounds. The production of antibacterial compounds might be influenced by nutrients (C and N sources) and incubation time. The aims of this study were to know the medium compositions (A: glucose 0.5%, starch 1%, yeast extract 0.2%; B: glucose 0.5%, starch 1%, sodium nitrate 0.2%; C: oatmeal 1%, yeast extract 0.2%; and D: oatmeal 1%, sodium nitrate 0.2%), with incubation times of (7, 14, and 21 days) and their interactions in producing the crude extract of antibacterial compounds. The main parameter was the crude extract weight, with the supporting parameters of glucose concentration, biomass dry weight, filtrate pH, filtrate inhibitory zone diameter, and extract inhibitory zone diameter. The result showed that the medium compositions and incubation times affected the produced crude extract and other supporting parameters. The highest crude extract weight productions were respectively shown by medium A (0.802 mg) with 21 days incubation time (0.640 mg), yet no interaction between them. Antibacterial compounds synthesis with 21 days incubation time and the use of inorganic nitr ogen further supports the formation of antibacterial compounds.