Optimisation of the background electrolyte for the determination of anions by capillary electrophoresis: application to sweet whey

Abstract

In capillary electrophoresis, the background electrolyte (BGE) composition is the key parameter for achieving the quantification of ions. This study is focused on the optimization of a BGE for anion separation (buffer, UV sensor, electroosmotic flow modifier and pH), and its application to whey anions. The quantification of chloride, sulfate, citrate and phosphate, was achieved with a relative standard deviation of about 1% for chloride and about 5-6% for the other anions, and compared to conventional one-by-one anion titration methods. © Inra/Elsevier, Paris.