Single-Photon Counting Detectors for the Visible Range Between 300 and 1,000 nm

Abstract

The use of light polarization properties in the analysis of fluorescence images has driven a large amount of research towards the measurement of orientational behavior of molecules in cells, in particular in their membranes. This field has been recently revisited to enlarge the possibilities of polarization resolved fluorescence microscopy. We show that this technique allows retreiving a wealth of information on the constraints that hinder rotational mobility of lipid probes and proteins in membranes, bringing thus new insights on inter-proteins and lipid-proteins interactions, on membrane morphology at the sub-diffraction length scale and on local membrane physical properties such as viscosity.