Structural elucidation of complex carbohydrates in solution is not a trivial task. From the NMR view point, the limited chemical shift dispersion of sugar NMR spectra demands the combination of a variety of NMR techniques as well as the employment of molecular modeling methods. Herein, a general protocol for assignment of resonances and determination of inter-proton distances within the saccharides by homonuclear and heteronuclear experiments (i.e., 1H and 13C) is described. In addition, several computational tools and procedures for getting a final ensemble of geometries that represent the structure in solution are presented.
CITATION STYLE
Díaz, D., Canales-Mayordomo, A., Javier Cañada, F., & Jiménez-Barbero, J. (2015). Solution conformation of carbohydrates: A view by using NMR assisted by modeling. Methods in Molecular Biology, 1273, 261–287. https://doi.org/10.1007/978-1-4939-2343-4_19
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