Behavior of pseudomonas aeruginosa and enterobacter aerogenes in water from filter jugs

Abstract

Careless use conditions of filter jugs were applied to simulate and evaluate the behavior of two ubiquitous aquatic bacterial species, Pseudomonas aeruginosa and Enterobacter aerogenes. According to a reference protocol, nine different jugs of popular brands sold in the Italian market were used for the test. Separately, a suspension of the two bacteria was spiked in water used for filling the jugs. The concentration of the test organisms and total aerobic microbial count (TAMC) was measured daily in the filtered water along a period corresponding to the cartridge lifetime. Results showed a different trend of bacterial behavior. E. aerogenes was detectable exclusively on the first day after jug filling, while P. aeruginosa confirmed its persistence over time in all the jugs and its ability to potentially colonize surfaces and cartridges. The TAMC was detected at a concentration range from 102 to 107 CFU/100 mL in all the tests, high values that were not far from those raised in bottled flat natural mineral water weeks after bottling.