Next-Generation Sequencing: Current Technologies and Applications

Abstract

"High-throughput, next-generation sequencing (NGS) technologies are capable of producing a huge amount of sequence data in a relatively short time and have revolutionized genome research in recent years. The powerful and flexible nature of NGS has made it an indispensable tool for a broad spectrum of biological sciences and NGS technologies have transformed scientific research in many fields. Written by experts from around the world, this book explores the most recent advances in NGS instrumentation and data analysis. The book begins with a comprehensive description of current NGS platforms, their sequencing chemistries, instrument specifications, and general workflows and procedures. A separate chapter is dedicated to low-quantity, single molecule sequencing technology. Further chapters explore the application of NGS technologies in various fields including polymorphism detection, sRNA research, rare variant detection, large variant detection, exome sequencing, plant development studies, microbial metagenomics, and studies on the human microbiome"--Publisher's description. An overview of next-generation genome sequencing platforms / Chandra Shekhar Pareek -- Attomole-level genomics with single-molecule direct DNA, cDNA and RNA sequencing technologies / Fatih Ozsolak -- SNP assessment on draft genomes from next generation sequencing data / Michael Piechotta and Christoph Dieterich -- Processing large-scale small RNA datasets in Silico / Daniel Mapleson, Irina Mohorianu, Helio Pais, Matthew Stocks, Leighton Folkes and Vincent Moulton -- Utility of high throughput sequence data in rare variant detection / Viacheslav Y. Fofanov, Tudor Constantin, Heather Koshinsky and Eureka Genomics -- Detecting break points of insertions and deletions from paired-end short reads / Kai Ye and Zemin Ning -- Novel insights from re-sequencing of human exomes through NGS / Jun Li, Tao Jiang, Xu Yang and Jun Wang -- Insights on plant development using NGS technologies / Ying Wang and Yuling Jiao -- Next generation sequencing and the future of microbial metagenomics / Andreas Wilke, Peter Larsen and Jack A Gilbert -- Next generation sequencing, metagenomes and the human microbiome / Karen E. Nelson, Ramana Madupu, Sebastian Szpakowski, Johannes Goll, Konstantinos Krampis and Barbara A. Methé.