Chicken tracheal organ cultures (TOCs), comprising transverse sections of chick embryo trachea with beating cilia, have proved useful in the isolation of several respiratory viruses and as a viral assay system, using ciliostasis as the criterion for infection. A simple technique for the preparation of chicken tracheal organ cultures in glass test tubes, in which virus growth and ciliostasis can be readily observed, is described. © 2008 Humana Press.
CITATION STYLE
Jones, B. V., & Hennion, R. M. (2008). The preparation of chicken tracheal organ cultures for virus isolation, propagation, and titration. Methods in Molecular Biology, 454, 103–107. https://doi.org/10.1007/978-1-59745-181-9_9
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