Micropropagation of Shinano walnut (Juglans regia L.)

Abstract

Micropropagation of three cultivars of Shinano walnut (Juglans regia L.) was performed by using DKW medium. Nodal segments (explants) produced new shoots on DKW medium supplemented with 5 μM BA and 0.05 μM IBA. When subcultured, shoots grew better on the above medium with agar than they did with Gelrite. There was a significant difference in the growth of shoots among the cultivars. To induce roots, IBA-treated shoots were transferred to a rooting medium, a Gelrite-solidified DKW medium with macroelements reduced to 1/4 strength, with or without vermiculite. The addition of vermiculite to the medium improved the rooting percentage and promoted the development of a good root system. All regenerated plantlets were successfully acclimatized.