Background: For next generation DNA sequencing, we have developed a rapid and simple approach for preparing DNA libraries of targeted DNA content. Current protocols for preparing DNA for next-generation targeted sequencing are labor-intensive, require large amounts of starting material, and are prone to artifacts that result from necessary PCR amplification of sequencing libraries. Typically, sample preparation for targeted NGS is a two-step process where (1) the desired regions are selectively captured and (2) the ends of the DNA molecules are modified to render them compatible with any given NGS sequencing platform.Results: In this proof-of-concept study, we present an integrated approach that combines these two separate steps into one. Our method involves circularization of a specific genomic DNA molecule that directly incorporates the necessary components for conducting sequencing in a single assay and requires only one PCR amplification step. We also show that specific regions of the genome can be targeted and sequenced without any PCR amplification.Conclusion: We anticipate that these rapid targeted libraries will be useful for validation of variants and may have diagnostic application. © 2011 Myllykangas et al; licensee BioMed Central Ltd.
CITATION STYLE
Myllykangas, S., Natsoulis, G., Bell, J. M., & Ji, H. P. (2011). Targeted sequencing library preparation by genomic DNA circularization. BMC Biotechnology, 11. https://doi.org/10.1186/1472-6750-11-122
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