Monoclonal antibody against a gill Rickettsialeslike organism of Pecten maximus (Bivalvia): Application to indirect immunofluorescence diagnosis

Abstract

Using hybridoma technology, monoclonal antibodies (MAB5) were prepared against a Rickettsiales-like organism (RLO) from the gills of the Saint-Jacques scallop Pecten niax.irnus. The 5D7 MAB was then selected as a specific reagent suitable for an indirect immunofluorescence assay (IIFA) and evaluated for RLO diagnosis on P. maximus gill smears. Monoclonal antibody 5D7 showed no cross- reactivity with an RLO associated with a related pectinid species Chlamys opercu]aris.