Synechococcus are distributed throughout the world's oceans and are composed of diverse genetic lineages. However, as they are much less abundant than Prochlorococcus in oligotrophic open oceans, their in-depth genetic diversity cannot be investigated using commonly used primers targeting both Prochlorococcus and Synechococcus. Thus, in this study, we designed a primer specifc to the 16S-23S rRNA internal transcribed spacer (ITS) of the Synechococcus subcluster 5.1. Using the primer, we could selectively amplify Synechococcus sequences in oligotrophic seawater samples. Further, we showed that a barcoded amplicon pyrosequencing method could be applicable to investigate Synechococcus diversity using sequences retrieved in GenBank and obtained from environmental samples. Allowing sequence analyses of a large number of samples, this high-throughput method would be useful to study global biodiversity and biogeographic patterns of Synechococcus in marine environments.
CITATION STYLE
Choi, D. H., Noh, J. H., & Lee, J. H. (2014). Application of pyrosequencing method for investigating the diversity of synechococcus subcluster 5.1 in Open Ocean. Microbes and Environments, 29(1), 17–22. https://doi.org/10.1264/jsme2.me13063
Mendeley helps you to discover research relevant for your work.