Response surface methodology optimization extraction of polysaccharides from maca (Lepidium meyenii) leaves and primary characterization

Abstract

Most of studies have focused on the maca (Lepidium Meyenii) hypocotyls, including the extraction, purification, characterization of bioactive components and their structures, but few on maca leaves. In this paper, we used response surface methodology to optimize the extraction conditions of maca leaves polysaccharides (MLPs). The optimal conditions were as follows: ratio of water to raw material (38 mL/g); extraction time, 150 min; extraction temperature, 100 °C and extraction times, 3. Under the condition, the experimental yield was 14.2 ± 0.25% (mL/g) (n = 3), which was close to the predicted value. The primary characterization of the crude MLPs was explored, and we carried on the FT-IR experiment. The FT-IR analysis revealed the general characteristic absorption peaks of the crude MLPs.