Case control feasibility study assessing the association between severity of coronary artery disease with Glutathione Peroxidase-1 (GPX-1) and GPX-1 polymorphism (Pro198Leu)

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Abstract

Background: Glutathione peroxidase-1 (GPX-1) activity was reported to be useful marker for monitoring cardiovascular disease. However, accurate assessment of coronary artery disease (CAD) using GPX-1 polymorphism is limited for South Asian population. Present study aim to assess GPX-1activity and GPX-1 polymorphismin patients with coronary artery disease (CAD) who were confirmed with coronary angiography findings and in apparently healthy subjects. Methods: Case control study was carried out with 85 patients (58 males and 27 females) 40-60 years of age confirmed as having CAD on coronary angiography findings and 85 age and sex matched healthy volunteers as controls. Blood samples were analyzed for erythrocyte GPX-1 activity and GPX-1 polymorphism in both groups and the severity of CAD was assessed using coronary angiography scoring system based on vessel, stenosis and extent score. Results: Coronary angiography scores indicated that erythrocyteGPX-1 cutoff value of 23.9 U/gHb showed a high sensitivity and negative predictive value in ruling out major vessel disease. The GPX-1 Pro198Leu (CT) polymorphism was higher in patients with CAD (25.3 %) when compared to controls (10.7 %). Pro198Leu (CT) genotype showed a 2.84 fold risk for CAD [odds ratio 2.84 (95 % CI 1.15-6.98), p = 0.019]. Conclusion: Coronary angiography findings indicated that individuals possessing Pro198Leu (CT) polymorphism were found to be associated with low erythrocyte GPX-1 activity and increased susceptibility for CAD.

Figures

  • Fig. 1 Amplified DNA by PCR. Extracted DNA amplified by PCR and resolved in 2 % agarose gel stained with ethidium bromide at 50 V for 2 h. Gel electrophoresis given 400 bp bands as target sequence
  • Fig. 2 Agarose gel electrophoresis image of restriction fragment length polymorphism. Amplified DNA by PCR was digested with the restriction enzyme 12 U of ApaI overnight at 37 °C and resolved in 2 % agarose gel stained with ethidium bromide at 50 V for 2 h. Resolved band patterns were photographed under ultra violet light by the Elite camera systems
  • Table 1 Demographic features and distribution of CAD risk factors in patients and controls
  • Table 2 Associations of risk models for coronary artery disease in study population by multivariate logistic regression analysis
  • Table 4 Frequency distributions of the three severityscore systems in patients with CAD
  • Table 3 Severity Scores of Coronary artery disease patients
  • Table 5 Receiver operating characteristic curves generated optimum cut-off values for coronary artery disease risk markers with severity of CAD scoring systems
  • Table 7 Association of GPX-1 Pro198leu polymorphism with severity of coronary artery disease

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APA

Wickremasinghe, D., Peiris, H., Chandrasena, L. G., Senaratne, V., & Perera, R. (2016). Case control feasibility study assessing the association between severity of coronary artery disease with Glutathione Peroxidase-1 (GPX-1) and GPX-1 polymorphism (Pro198Leu). BMC Cardiovascular Disorders, 16(1). https://doi.org/10.1186/s12872-016-0280-9

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