Optimizing rice-fish co-culture: Investigating the impact of rice spacing density on biochemical profiles and production of genetically modified tilapia (Oreochromis spp.) and Cyprinus carpio

Abstract

Rice fish co-culture synergistically boosts food production, resulting in numerous advantages across the environmental, social, and financial domains. A study was conducted to investigate the effects of three different rice spacing densities, rice high density (RHD) 9inch×12inch, rice medium density (RMD) 12inch × 12inch, rice low density (RLD) 15inch×12inchon both rice and fish. Various parameters were assessed to evaluate the performance of the co-culture system, including water quality, growth parameters, muscle quality, soil characteristics, rice stem characteristics, and rice yield parameters. When comparing the species, it was observed that GMT (Genetically Male Tilapia) demonstrated superior weight gain (303.13g vs 296.41g) and specific growth rate (1.16 vs 1.15). Regarding the proximate composition, results showed that RMD had the highest crude protein and fat content compared to RLD and RHD. GMT also exhibited greater crude protein and fat content than Cyprinus carpio, with RMD showing the highest values. Treatment groups significantly influenced the amino acid profile of experimental species, with RMD exhibiting the highest values. GMT showed significantly higher levels of essential, non-essential, half-essential, and umami amino acids compared to Cyprinus carpio. The interaction between RMD and GMT further demonstrated significant differences in various amino acid categories with RMD. A non-significant difference was observed among the treatments regarding soil biochemical characteristics. Regarding the rice stem characters, the height of the plant, panicle length, and stem length of rice were found to be comparable in the RMD and RLD groups however, significantly higher in RHD. Regarding rice yield parameters, no significant differences were observed among the other treatment groups, except for yield per hectare (yield/ha), which was significantly higher in the RHD group compared to RMD and RLD. Additionally, 1000-grain weight and panicle number (ears per hill) were significantly higher in the RLD treatment than in the other treatments. In conclusion, our findings indicate that the RMD treatment consistently yielded superior results compared to RLD and RHD. Furthermore, within the rice-fish co-culture system, GMT proved to be a more competent species compared to Cyprinus carpio. The study provides data to understand the interactions between rice spacing density, fish growth and overall productivity can guide the development of sustainable and profitable rice-fish co-culture systems.