Matrix-assisted laser desorption/ionization time-of-fl ight mass spectrometry (MALDI-TOF MS) has been recently introduced to many diagnostic microbiological laboratories. Besides the identifi cation of bacteria and fungi, that technique provides a potentially useful tool for the detection of antimicrobial resistance, especially of that conferred by β-lactamases. Here, we describe an assay allowing a detection of meropenem hydrolysis in clinical isolates of Enterobacteriaceae, Pseudomonas spp., and Acinetobacter baumannii using MALDI-TOF MS. This method is able to confi rm carbapenemases within 3 h. The results are important for proper and fast intervention to limit the spread of carbapenemase-producing bacteria and provide information for appropriate initial therapy of the infections caused by these microbes.
CITATION STYLE
Hrabák, J. (2015). Detection of carbapenemases using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (Maldi-tof ms) meropenem hydrolysis assay. Methods in Molecular Biology, 1237, 91–96. https://doi.org/10.1007/978-1-4939-1776-1_9
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