Evaluation of GenoType NTM-DR assay for identification of Mycobacterium chimaera

21Citations
Citations of this article
30Readers
Mendeley users who have this article in their library.

Abstract

Identification of species within the Mycobacterium avium complex (MAC) is difficult, and most current diagnostic laboratory tests cannot distinguish between species included in the complex. Differentiation of species within the MAC is important, as Mycobacterium chimaera has recently emerged as a major cause of invasive cardiovascular infections following open heart surgery. A new commercial diagnostic assay, GenoType NTM-DR ver. 1.0, is intended to differentiate between three species within the MAC, namely, Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium chimaera. In this study, we investigated an archival collection of 173 MAC isolates using 16S rRNA and 16S-23S internal transcribed spacer (ITS) gene sequencing, and GenoType NTM-DR was evaluated for identifying M. chimaera and other species belonging to the MAC. Species identification of 157/173 (91%) isolates with the GenoType NTM-DR assay was in agreement with 16S rRNA and 16S-23S ITS gene sequencing results. Misidentification occurred with 16 isolates which belonged to four species included in the MAC that are rarely encountered in clinical specimens. Despite some limitations of this assay, GenoType NTM-DR had 100% specificity for identifying M. chimaera. This novel assay will enable diagnostic laboratories to differentiate species belonging to the Mycobacterium avium complex and to accurately identify M. chimaera. It can produce rapid results and is also more cost efficient than gene sequencing methods.

References Powered by Scopus

5015Citations
2438Readers
Get full text

This article is free to access.

This article is free to access.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Mok, S., Rogers, T. R., & Fitzgibbon, M. (2017). Evaluation of GenoType NTM-DR assay for identification of Mycobacterium chimaera. Journal of Clinical Microbiology, 55(6), 1821–1826. https://doi.org/10.1128/JCM.00009-17

Readers over time

‘17‘18‘19‘20‘21‘22‘23‘2402468

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 8

53%

Researcher 5

33%

Professor / Associate Prof. 1

7%

Lecturer / Post doc 1

7%

Readers' Discipline

Tooltip

Medicine and Dentistry 7

37%

Biochemistry, Genetics and Molecular Bi... 5

26%

Immunology and Microbiology 5

26%

Agricultural and Biological Sciences 2

11%

Article Metrics

Tooltip
Social Media
Shares, Likes & Comments: 18

Save time finding and organizing research with Mendeley

Sign up for free
0