Calenduloside E 6'-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 xenograft animal model

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Abstract

The aim of the present study was to investigate the cytotoxic effect of calenduloside E 6'-methyl ester (oleanolic acid 3-O-β-D-glucuronopyranoside-6'-methyl ester) isolated from Acanthopanax sessiliflorus fruits was investigated in CT-26 mouse colon carcinoma cells. Calenduloside E 6'-methyl ester dose-dependently inhibited the viability of CT-26 cells. Apoptosis was characterized by the detection of annexin-V and sub-G1 apoptotic cell populations, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and DNA fragmentation experiments. Results showed that the number of immunostained annexin-V-FITC and sub-G1 cells increased after treatment with calenduloside E 6'-methyl ester. Calenduloside E 6'-methyl ester also increased terminal deoxynucleotidyl transferase dUTP nick end labeled-CT-26 cells. It induced DNA fragmentation. and the cleavage of caspase-8, -9, -3 and poly ADP-ribose polymerases. In addition, calenduloside E 6'-methyl ester suppressed the volume and weight of tumors in BALB/c mice subcutaneously implanted with CT-26 cells. These results indicate that calenduloside E 6'-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 carcinoma animal model.

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Lee, B., Lee, D. Y., Yoo, K. H., Baek, N. I., Park, J. H., & Chung, I. S. (2012). Calenduloside E 6’-methyl ester induces apoptosis in CT-26 mouse colon carcinoma cells and inhibits tumor growth in a CT-26 xenograft animal model. Oncology Letters, 4(1), 22–28. https://doi.org/10.3892/ol.2012.693

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