Study of growth patterns of staphylococcus aureus in human blood and sheep

Abstract

This study aims to observe the growth pattern of Staphylococcus aureus on BAP media O, AB and sheep blood types as control. The purpose of this study was to test the feasibility of using human blood groups O and AB as important compounds in the manufacture of sheep BAP as a substitute for blood media. In this experiment, Blood Agar Plate (BAP) was tested using sheep blood as a substitute medium for human blood, which is used to identify and isolate the Staphylococcus aureus bacterium. The growth pattern of Staphylococcus aureus was observed macroscopically against colony morphology, the number of colonies, and the diameter of the hemolysis zone. Suspension of Staphylococcus aureus (ATCC 25923) using pure culture synchronized with McFarland's 0.5 Standard solutions. The depletion is then carried out 10. Suspension of bacteria that have undergone depletion is planted in BAP media O, AB and sheep blood groups are filled in each of the 9 Petri dishes. The results of observations are known colony morphology, some colonies, and hemolysis zone diameters and statistical tests using the Kruskal Wallis test values obtained by Asymp. Sig of 0.352 for the number of colonies and the diameter of the hemolysis zone is greater than α (0.05), which means there is no significant difference between the growth patterns of Staphylococcus aureus on BAP media using human blood groups O, AB and sheep blood as BAP media control.