X-ray diffraction analysis was done on the membrane couplets isolated from newborn mouse stratum corneum. The same lipid reflections were observed for whole stratum corneum and couplets, adding further support to the thesis that stratum corneum lipid is intercellular in location rather than associated with the intracellular filamentous protein.
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Elias, P. M., Bonar, L., Grayson, S., & Baden, H. P. (1983). X-ray diffraction analysis of stratum corneum membrane couplets. Journal of Investigative Dermatology, 80(3), 213–214. https://doi.org/10.1111/1523-1747.ep12534362