Plant Gene Transfer and Expression Protocols

Abstract

Tools for expressing foreign genes in plants / François Guerineau -- Introduction of cloning plasmids into Agrobacterium tumefaciens / Charles H. Shaw -- Leaf disk transformation using Agrobacterium tumefaciens : expression of heterologous genes in tobacco / Patrick Gallois, Paulo Marinho -- Agobacterium rhizogenes as a vector for transforming higher plants : application in Lotus corniculatus transformation / Jens Stougaard -- Agrobacterium-mediated transformation of Arabidopsis thaliana : application in T-DNA tagging / Jennifer F. Topping, Wenbin Wei, Michael C. Clarke, Paul Muskett, Keith Lindsey -- Agrobacterium-mediated transfer of geminiviruses to plant tissues / Margaret I. Boulton -- Stable transformation of barley via direct DNA uptake : electroporation- and PEG-mediated protoplast transformation / Paul A. Lazzeri -- Gene transfer into plant protoplasts by electroporation / Heddwyn Jones -- Transformation of cereals by microprojectile bombardment of immature inflorescence and scutellum tissues / Pilar Barcelo, Paul A. Lazzeri -- The [beta]-glucuronidase (gus) reporter gene system : gene fusions : spectrophotometric, fluorometric, and histochemical detection / Gillian A. Hull, Martine Devic -- Chloramphenicol acetyl transferase assay / Michael R. Davey, Nigel W. Blackhall, J. Brian Power -- NPTII assays for measuring gene expression and enzyme activity in transgenic plants / Ian S. Curtis, J. Brian Power, Michael R. Davey -- Gene characterization by Southern analysis / Paolo A. Sabelli, Peter R. Shewry -- Isolation and characterization of plant genomic DNA sequences via (inverse) PCR amplification / Remko Offringa, Frederique Lee -- Isolation of whole cell (total) RNA / Rod J. Scott -- Poly(A)+ RNA Isolation / Rod J. Scott -- In vitro translation / Rod J. Scott -- Northern analysis and nucleic acid probes / Paolo A. Sabelli, Peter R. Shewry -- Nuclear "run-on" transcription assays / John Gatehouse, Andrew J. Thompson -- RNase A/T1 protection assay / Craig G. Simpson, John W. Brown -- Primer extension assay / Craig G. Simpson, John W. Brown -- Applications of RT-PCR / Craig G. Simpson, John W. Brown -- In vitro transcription of Class II promoters in higher plants / Richard Cooke, Paul Penon -- Analysis of plant gene expression by reverse transcription-pcr / Mark Bell -- In situ hybridization to plant tissue sections / Shirley R. Burgess -- Xenopus oocytes as a heterologous expression system / Frederica L. Theodoulou, Anthony J. Miller -- Heterologous expression in yeast / Laurence J. Trueman -- The isolation of intact chloroplasts / Johnathan A. Napier, Simon A. Barnes -- In vitro protein import by isolated chloroplasts / Johnathan A. Napier -- Targeting of foreign proteins to the chloroplast / Johnathan A. Napier -- Isolation of mitochondria / Stefan Binder, Lutz Grohmann -- Mitochondrial nucleic acid purification and analysis / Stefan Binder -- In organello protein synthesis / Lutz Grohmann -- Separation of plant proteins by electrophoresis / Peter R. Shewry, Arthur S. Tatham, Roger J. Fido -- Western blotting analysis / Roger J. Fido, Arthur S. Tatham, Peter R. Shewry -- ELISA detection of foreign proteins / E.N. Mills, Geoffrey W. Plumb, Michael R. Morgan -- Immunocytochemical localization of proteins / John Davies.