Abstract
The use of in vivo NMR within the framework of Metabolic Flux Analysis in plants is presented. In vivo NMR allows to visualize the active metabolic network, to determine metabolic and isotopic steady state and to measure metabolic fluxes which are not necessarily accessible by isotopic steady state (stationary) Metabolic Flux Analysis. The kinetic data can be used as input for dynamic (nonstationary) Metabolic Flux Analysis. Both 1D and 2D NMR methods are employed. © Springer Science+Business Media New York 2014.
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Roscher, A., Troufflard, S., & Taghki, A. I. (2014). In vivo NMR for 13C metabolic flux analysis. Methods in Molecular Biology, 1090, 143–152. https://doi.org/10.1007/978-1-62703-688-7_10
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