Role of matrix metalloproteinases 7 in the pathogenesis of laryngopharyngeal reflux: Decreased e-cadherin in acid exposed primary human pharyngeal epithelial cells

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Abstract

Cleavage of E-cadherin and the resultant weakness in the cell-cell links in the laryngeal epithelium lining is induced by exposure to acidic contents of the refluxate. Herein, we aimed to evaluate the role of matrix metalloproteinases (MMPs) in inducing E-cadherin level changes following acid exposure to the human pharyngeal mucosal cells. E-cadherin levels were inversely correlated with the duration of acid exposure. Treatment with actinonin, a broad MMP inhibitor, inhibited this change. Immunocytochemical staining and transepithelial permeability test revealed that the cell surface staining of E-cadherin decreased and transepithelial permeability increased after acid exposure, which was significantly inhibited by the MMP inhibitor. Among the various MMPs analyzed, the mRNA for MMP-7 in the cellular component was upregulated, and the secretion and enzymatic activity of MMP-7 in the culture media increased with the acid treatment. Consequently, MMP-7 plays a significant role in the degradation of E-cadherin after exposure to a relatively weak acidic condition that would be similar to the physiologic condition that occurs in Laryngopharyngeal reflux disease patients.

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Im, N. R., Lee, D. Y., Kim, B., Kim, J., Jung, K. Y., Kim, T. H., & Baek, S. K. (2019). Role of matrix metalloproteinases 7 in the pathogenesis of laryngopharyngeal reflux: Decreased e-cadherin in acid exposed primary human pharyngeal epithelial cells. International Journal of Molecular Sciences, 20(21). https://doi.org/10.3390/ijms20215276

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