Fungal assembly of L-asparaginase using solid-state fermentation: A review

Abstract

Because of its antitumor therapeutic-activity, as well as its application in food industries to improve the quality, L-asparaginase has attracted considerable attention from several investigators. In recent years, fungi have occupied advanced rank among microorganisms in the production process of the enzyme. This review is spotting the light on the advantages of fungal enzyme and its applications in the food industry and medications. The solid-state fermentation was discussed as the wide alternative and most accepted biosynthesis technique. However, some lights were also spotted to the statistical experimental design of the fermentation process, mainly on the methodology of the response surface for L-asparaginase biosynthesis by fungi. Finally, the immobilization of the enzyme and the features of the widely used solid substrates for the maximization of the production process were explored.