Human neutrophils stimulated with either latex particles or opsonized zymosan exhibited equivalent rates of net oxygen consumption as well as hydrogen peroxide release. The quantity of superoxide (02¯) detected in latex-stimulated neutrophils was less than 2% of that seen with opsonized zymosan stimulation, and only several-fold greater than that of resting cells. The failure to detect O2¯ in the latex-stimulated neutrophils was due neither to latex acting as a O2¯ scavenger nor to its interference with the O2¯ forming system of the neutrophil. An intracellular site of O2¯generation could not be demonstrated. NADPH oxidase activity in cells exposed to latex particles was only 10% of that seen in cells comparably activated with opsonized zymosan. Latex particles have the unusual property of stimulating the respiratory burst of the human neutrophil without the extracellular release of O2¯. The potential physiologic importance of this finding is discussed. © 1983 International Pediatric Research Foundation, Inc.
CITATION STYLE
Curnutte, J. T., & Tauber, A. I. (1983). Failure to detect superoxide in human neutrophils stimulated with latex particles. Pediatric Research, 17(4), 281–284. https://doi.org/10.1203/00006450-198304000-00011
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