Tub-tag labeling, a novel chemoenzymatic protein functionalization method, facilitates one-step fluorescent labeling of functional biomolecules. The enzyme tubulin tyrosine ligase incorporates coumarin-amino acids to the terminal carboxylic acid of proteins containing a short peptidic recognition sequence called Tub-tag. Here we describe the one-step Tub-tag protein modification protocol in detail and explain its utilization to generate fluorescently labeled proteins for advanced applications in imaging and diagnostics.
CITATION STYLE
Schumacher, D., Leonhardt, H., Hackenberger, C. P. R., & Helma, J. (2019). One-Step Fluorescent Protein Labeling by Tubulin Tyrosine Ligase. In Methods in Molecular Biology (Vol. 2033, pp. 167–189). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9654-4_12
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