We previously isolated Aurora-C (Aurkc/Aie1) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment, segregation, kinetochore-microtubule attachment, and cytokinesis in female mouse meiosis. This chapter describes experimental approaches for examining the subcellular localization and function of Aurora-C kinase during female mouse meiosis, presenting detailed methods for introducing exogenous Aurora-C wild-type and kinase-dead mutant mRNAs into mouse oocytes by cytosolic microinjection, and preparing whole-mount meiotic oocytes and chromosome spreads for confocal immunofluorescence microscopy. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Yang, K. T., Lin, Y. N., Li, S. K., & Tang, T. K. (2013). Studying the roles of aurora-C kinase during meiosis in mouse oocytes. Methods in Molecular Biology, 957, 189–202. https://doi.org/10.1007/978-1-62703-191-2_13
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