Abstract
Piwi interacting RNAs (piRNAs) are small (∼25 to ∼30 nucleotide) and are expressed in the germline. piRNAs bind to the Piwi subclade of Argonaute proteins and form the core ribonucleoproteins (RNPs) of piRNPs. We describe a method for the massive identification of piRNAs from immunopurified piRNPs. This strategy may also be used for immunopurification and directional sequencing of RNAs from other RNPs that contain small RNAs.
Author supplied keywords
Cite
CITATION STYLE
Kirino, Y., Vourekas, A., Khandros, E., & Mourelatos, Z. (2011). Immunoprecipitation of piRNPs and Directional, Next Generation Sequencing of piRNAs. In Methods in Molecular Biology (Vol. 725, pp. 281–293). Humana Press Inc. https://doi.org/10.1007/978-1-61779-046-1_18
Register to see more suggestions
Mendeley helps you to discover research relevant for your work.
