The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord.
CITATION STYLE
Koike, Y., Li, B., Chen, Y., Miyake, H., Lee, C., Chi, L., … Pierro, A. (2018). Live imaging of fetal intra-abdominal organs using two-photon laser-scanning microscopy. In Methods in Molecular Biology (Vol. 1752, pp. 63–69). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7714-7_6
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