The polymorphism in interleukin-6-597 G/A gene and their levels on type 2 diabetic patients

Abstract

BACKGROUND: Interleukin-6 (IL-6) pro-inflammatory cytokines play a role in the pathogenesis of inflammatory reactions in type 2 diabetes mellitus (T2DM). The-597G/A is one of IL-6 gene polymorphisms that are associated with the T2DM risk. AIM: This study aimed to observe the polymorphism in IL-6-597 G/A gene and their levels on type 2 diabetic patients at Universitas Sumatera Utara Hospital. MATERIALS AND METHODS: IL-6-597 G/A gene polymorphisms and levels were done in 80 type 2 diabetic patients. The levels of IL-6 were performed by enzyme-linked immunosorbent assay method. Analysis of IL-6-597 G/A gene polymorphism was done using polymerase chain reaction (PCR) and restriction fragment length polymorphism. The PCR products were cut by FokI restriction enzyme and then visualized with 4% agarose. RESULTS: This study showed that the genotype frequency of GG and GA was 97.5 % and 2.5 %, respectively; however, no A/A genotype shown in this population. IL-6 levels were higher in GG genotype group compare to GA+AA genotype group, with the association were significant (p < 0.05). CONCLUSION: This study indicated that GG genotype was common genotype in the IL-6-597 G/A gene polymorphism and the polymorphism was significantly with the IL-6 levels.