High avidity, pentameric, single-domain antibodies, oligomerized through the B subunit of verotoxin, are excellent immunohistochemical reagents. The resulting molecules are termed pentabodies. Here, we describe the immunostaining of tissue sections with ES1, a pentabody recognizing CEACAM6 which is overexpressed in several cancers. The advantages of pentabodies, compared to conventional antibody reagents, in immunohistochemical studies are highlighted. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Maik, K. T., & MacKenzie, C. R. (2012). Application of single-domain antibodies in tumor histochemistry. Methods in Molecular Biology, 911, 523–530. https://doi.org/10.1007/978-1-61779-968-6_33
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