Caries Experience among Females aged 16–21 in Punjab, India and its Relationship with Lifestyle and Salivary HSP70 Levels

Abstract

Objectives: The objective of the current study was to assess the prevalence and means of dental caries experience and the prevalence of calculus among college students and their awareness of general dental health. The correlation between the experience of caries and extracellular levels of heat shock protein-70 (eHSP70) was studied in saliva samples of these students to ascertain whether eHSP level changes as a result of increased caries experience of these subjects.Methods: The current study was conducted among 147 female undergraduate students aged 16– 21 in the state of Punjab, India. Dental examinations were performed to assess for the presence of calculus and DMFT, and subjects were asked to fill in a questionnaire. The data were recorded and analysed for the role of various factors in the prevalence of caries, including dietary habits. Saliva samples were collected for ELISA-based assay of eHSP70 levels.Results: The prevalence of caries experience (DMFT>0) was 63.2%, with average DMFT of 2.91 in students aged 16–18 and 3.26 in those aged 19–21. Of those with caries, 68% also showed the presence of calculus, compared with 60.0% of subjects with DMFT=0. The level of eHSP was significantly higher (P