Mass western for absolute quantification of target proteins and considerations about the instrument of choice

Abstract

The Mass Western describes the absolute quantification of proteins based on stable isotope labeled integral standard peptides and liquid chromatography coupled selective reaction monitoring triple quadrupole mass spectrometry (LC-SRM/MS). Here, we present a detailed workflow including tips and we discuss advantages and disadvantages of using different types of MS for absolute quantification. © 2014 Springer Science+Business Media, LLC.