Comparative in Silico evaluation of MYB transcription factors in eucalyptus, sugarcane and rice transcriptomes

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Abstract

Transcription factors are essential in plant growth and development regulation and during response activation to environmental stimuli. This study examined 237,954 sugarcane and 110,000 eucalyptus ESTs in search of genes related to MYB factors active during abiotic stresses in arabidopsis and rice. Searches revealed relative abundance and diversity of MYB candidate sequences in both transcriptomes. In general, 71 and 42 MYB sequences have been selected in eucalyptus and sugarcane, respectively. The generated phenogram, using selected sequences and sequences from other organisms, revealed a sub-grouping according to domain-repeats number; confirming the ancestral origin of MYB genes. Expression pattern analysis revealed higher number of reads in wood, flower and bark libraries in eucalyptus, and in tissues infected by pathogens in sugarcane. Splicing pattern comparative analyses of arabidopsis and rice ORFs with eucalyptus and sugarcane ORFs revealed that neither of them presented similarities regarding all exons and intron junctions. © 2009 Springer Berlin Heidelberg.

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Soares-Cavalcanti, N. M., Wanderley-Nogueira, A. C., Belarmino, L. C., Dos Santos Barros, P., & Benko-Iseppon, A. M. (2009). Comparative in Silico evaluation of MYB transcription factors in eucalyptus, sugarcane and rice transcriptomes. In Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) (Vol. 5488 LNBI, pp. 44–55). https://doi.org/10.1007/978-3-642-02504-4_4

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