Eukaryotic cells comprise various organelles surrounded by the membrane. Each organelle is characterized by unique proteins and lipids and has its own specific functions. Single membrane-bounded organelles, including the Golgi apparatus, endosomes, and vacuoles are connected by membrane trafficking. Identifying the organelle localization of a protein of interest is essential for determining the proteins physiological functions. Here, we describe methods for determining protein subcellular localization using the inhibitors brefeldin A and wortmannin in Arabidopsis thaliana.
CITATION STYLE
Takagi, J., & Uemura, T. (2018). Use of brefeldin a and wortmannin to dissect post-golgi organelles related to vacuolar transport in arabidopsis thaliana. In Methods in Molecular Biology (Vol. 1789, pp. 155–165). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7856-4_12
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