UPLC-MS is a commonly used technique to first separate complex samples and subsequently quantify molecules of interest. Herein we describe the use of UPLC-MS using an amide stationary phase to quantify non-derivatized amino acids extracted from fingerprints. As detector either a triple-quadrupole MS/MS or a TOF-MS detector was used. This method allows for a simple and fast sample preparation, which facilitates the analysis of large amounts of samples.
CITATION STYLE
van Helmond, W., & de Puit, M. (2019). Amino Acid Profiling from Fingerprints Using Amide Stationary-Phase UPLC-MS. In Methods in Molecular Biology (Vol. 2030, pp. 439–450). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9639-1_33
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