Molecular architecture and functional analysis of NetB, a pore-forming toxin from Clostridium perfringens

Abstract

Background: Clostridium perfringens toxin NetB is a key factor in avian necrotic enteritis. Results: NetB forms heptameric pores structurally similar to Staphylococcus aureus toxins but lacks a phosphocholine binding pocket. NetB activity is enhanced by cholesterol. Conclusion: NetB has distinct binding specificity, and cholesterol may act as a receptor. Significance: The structure of NetB will facilitate development of control measures against necrotic enteritis. © 2013 by The American Society for Biochemistry and Molecular Biology, Inc.