The construction and screening of metagenomic expression libraries have a great potential to identify novel genes with desired functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.
CITATION STYLE
Mahmud, B., Boolchandani, M., Patel, S., & Dantas, G. (2023). Functional Metagenomics to Study Antibiotic Resistance. In Methods in Molecular Biology (Vol. 2601, pp. 379–401). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2855-3_21
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