Next-generation sequencing of noncoding RNA (ncRNA) libraries has become an essential tool for the profiling of ncRNAs and the identification of novel ncRNA species. Here, we describe the generation of a ncRNA-derived complementary DNA (cDNA) library by 3′-tailing of ncRNAs by CTP and poly(A) polymerase, followed by 5′-adapter ligation by T4 RNA ligase and reverse transcription of ncRNAs with an oligo-d(G) anchor primer. Preliminary selection of ncRNAs from ribonucleoprotein particles (RNPs) enables a strong enrichment of the generated libraries with functional regulatory ncRNAs compared to classical approaches. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Rederstorff, M. (2012). Generation of cDNA libraries from RNP-derived regulatory noncoding RNAs. Methods in Molecular Biology, 925, 211–218. https://doi.org/10.1007/978-1-62703-011-3_14
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