Microtubule plus-tip tracking is a powerful method to measure microtubule growth dynamics in vivo. Here we outline an approach that exploits live confocal microscopy of a GFP-tagged EB1-like protein to measure microtubule growth behavior and minus-end-directed microtubule motor activity at the cortex of Caenorhabditis elegans embryos. The EB1 velocity assay (EVA) provides a method to reproducibly monitor motor- and non-motor-assisted microtubule movements.
CITATION STYLE
Tegha-Dunghu, J., Gusnowski, E. M., & Srayko, M. (2014). Measuring microtubule growth and gliding in caenorhabditis elegans embryos. Methods in Molecular Biology, 1136, 103–116. https://doi.org/10.1007/978-1-4939-0329-0_7
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