Interleukin-17 is produced by both Th1 and Th2 lymphocytes, and modulates interferon-γ- and interleukin-4- induced activation of human keratinocytes

263Citations
Citations of this article
93Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Interleukin-17 is a T-cell-derived cytokine, detected in skin affected by allergic contact dermatitis and psoriasis, which regulates keratinocyte expression of adhesion molecules and chemokines. In this study, we have analyzed whether interleukin-17 production segregates with a particular T helper (Th) cell subset, and have examined the capacity of interleukin-17 to modulate the activation of keratinocytes induced by Th1 and Th2 cytokines. A panel of 80 nickel-specific CD4+ T cell clones (36 Th0, 30 Th1, and 14 Th2) was isolated from peripheral blood or lesional skin of allergic contact dermatitis patients. Significant amounts (>50 pg per ml) of interleukin-17 were released by about 50% of activated Th0, Th1, and Th2 cells. Interleukin- 17 alone and in cooperation with interleukin-4, or to a lesser extent with interferon-γ, decreased the interleukin-1 receptor antagonist to interleukin-1α ratio in the supernatants as well as in cell lysates from keratinocytes. In addition, interleukin-17 stimulated the release of growth- regulated oncogene-α, granulocyte-macrophage colony stimulating factor, and interleukin-6, with synergistic or additive effects when used together with interferon-γ or interleukin-4. Interleukin-17 and interleukin-4 also increased stem cell factor release, a function that was inhibited by interferon-γ. Moreover, interleukin-17 and interleukin-4 enhanced interferon-γ-induced expression of intercellular adhesion molecule 1, but not CD40, on keratinocytes. The constitutive expression of interleukin-17 and interferon-γ receptors on keratinocytes was not modulated by interleukin-17, interferon-γ, or interleukin-4, whereas the interleukin-4 receptor was significantly downregulated by interferon-γ. As a whole, the results indicate that interleukin-17 can participate relevantly in T-cell-mediated skin immune responses by amplifying both interferon-γ- and interleukin-4- induced activation of keratinocytes.

Cite

CITATION STYLE

APA

Albanesi, C., Scarponi, C., Cavani, A., Federici, M., Nasorri, F., & Girolomoni, G. (2000). Interleukin-17 is produced by both Th1 and Th2 lymphocytes, and modulates interferon-γ- and interleukin-4- induced activation of human keratinocytes. Journal of Investigative Dermatology, 115(1), 81–87. https://doi.org/10.1046/j.1523-1747.2000.00041.x

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free