IN VITRO AND IN SILICO ANTIBACTERIAL ACTIVITY OF PRANGOS¬ FERULACEA (L.) Lindl AND PRANGOS ULOPTERA DC, AND THEIR MUTAGENICITY IN THE AMES TEST

Abstract

The present study was conducted to study antibacterial activity of different extracts of Prangos ferulacea and Prangos uloptera. The antibacterial activity was measured by disc diffusion and micro-broth dilution methods at different concentrations (250, 500, 750, 1000, 1500, 2000, 2500 and 3000μg/ml).The studied bacteria were Streptococcus pyogenes, Staphylococcus aureus, Bacillus subtilis, Serratia marcescens, Escherichia coli, Salmonella enterica.The in sillico antibacterial activity of pinens and coumarins was performed by Autodock 4 software. The molecular docking between phytochemicals and six target proteins (DNAgyrase subunit B, pinicilin binding protein, D-alanin D-alanin syntase, dihydrofolate reductase, and dihydropteroate synthetase and isoleucyl-tRNA sinthetase) has been investigated. The mutagenicity of these extracts at different concentrations (500, 1000, 1500, 2000, 2500, 3000µg/ml) were also investigated on Salmonella typhimurium strain TA98. The results confirmed that all tested extracts have modest to weak antibacterial activity against studied bacteria without any mutagenicity effect.The root and seed extracts of both species respectively had highest and lowest antibacterial effects.The antibacterial activity of pinens of these plants was significantly more than coumarins. DNA gyrase subunit B and penicilin binding proteins (PBP) were the main targets of tested coumarins. DNA gyrase subunit B was also the main target of studied pinens.Our study found that P.ferulacea and P.uloptera displayed a great potential of antibacterial activity.