Screening of synergistic and antimicrobial effect of cr (Vi) and ni (ii) tolerant bacteria bacillus cereus

Abstract

The East Kolkata Wetland is a combination of natural and human made area combining ponds, and agricultural lands, garbage disposal area containing pollutants such as heavy metal, oil, grease, and solid wastes. Nowadays, wetland assessment often lack of an in-depth analysis of soil parameters and factors affecting the agricultural development. Primarily this work considers the analysis of soil samples for key parameters such as temperature, water holding capacity, pH, measurement of electrical conductivity, determination of Organic Carbon, Nitrogen and Phosphorus, and presence of heavy metals chromium (VI) and nickel (II), whereas the main focus of this work is the reduction activity of the disposed of heavy metals (Cr and Ni) with bacterial source and to check the synergistic effect of bacterial resistance against both the heavy metals. Bacterial resistant capacity was checked against different concentrations of metals using agar plate and reduction was observed using different electron donor source. Further, the identified strains were also checked against different microbes for their antimicrobial activity. The isolates BRS 11 and BRS 17 showed a good reduction and accumulation capacity against chromium and nickel in the presence of electron donors. Furthermore, the statistical analysis showed positive significance between each other confirming the synergistic effects.