Pseudomonas fluorescens Isolates Used as a Plant Growth Promoter of Faba Bean (Vicia faba) in Vitro as Well as in Vivo Study in Ethiopia

Abstract

Production of the crop is affected by deficiency of fertilizers and low number of plant growth promoting rhizobacteria in soil. At the present study, Pseudomonas fluorescens isolates possess a variety of promising properties which make it a good plant growth promoting traits. Twelve P. fluorescens isolates from rhizospheric soil of faba bean were isolated and assessed in vitro for their plant growth promoting activity based on their ability to produce hydrogen cyanide (HCN), siderophores, indole acetic acid (IAA), and ammonia and phosphate solubilization. The results indicated that most of the isolates tested possess plant growth promoting traits. Bio-primed faba bean seed with P. fluorescens 9 and P. fluorescens10 for plant growth promoting activities in green house showed a positive result. In addition, these to two isolates increased faba bean leaves number, branches number, height, root length, lateral roots and number of nodule per plant. Therefore, from this study it is possible to conclude that the use of P. fluorescens 9 and 10 isolates could increase the faba bean growth and yield performance. These isolates can be used as potential biofertilizers and plant growth promoter.