Newt lens transdifferentiation: From lentectomy to immuno-fish

Abstract

Newt lens regeneration is achieved by a unique cellular regulation of transdifferentiation where the dorsal iris pigmented epithelial cells (PECs) dedifferentiate and redifferentiate into lens cells. Recent studies have shown that nuclear architecture of PECs is dynamically changed and unique epigenetic regulation in somatic nucleus is crucial in the lens transdifferentiation. Immuno-FISH, detection of protein and gene loci in nucleus, is one of the effective tools to analyze nuclear architecture of PECs. In this chapter a whole process from lentectomy to immuno-FISH is described.